%image subtraction in 1 image by 128
clear all;
close all;
%image histogram original image
image1 = imread('8.jpg');
Img1=rgb2gray(image1);
Img2=imsubtract(Img1,128);
%Img2=uint8(double(Img1)-128);
subplot(2,1,1)
imshow(Img1);
subplot(2,1,2)
imshow(Img2);